This study was carried out to examine the optimal cultural condition in enzyme activities of CMCase, FPase and xylanase in selected strains which secret extracellular enzymes for using deinking agent to old newsprint. The results of this study were as follow: The production of enzyme by Bacillus pumilus I was maximal as grown on the medium, containing of rice bran+xylan $2.0\%$, peptone $0.8\%,;K_2HPO_4;0.1\%;and;CaCl_2;0.06\%$ at pH 8.0 and $28^{circ}C$ for 72 hours. Optimal cultural condition of B. subtilis I was avicel+xylan $3.5\%,;urea;0.4\%,;K_3PO_4;0.1\%;and;CaCl_2;0.015\%$ at pH 9.0 and $28^{circ}C$ for 36 hours. The maximal enzyme production was observed in the medium, containing of avicel+xylan $3.5\%,;urea;1.6\%;and; K_2HPO_4;0.125\%$ with pH 9.0 when B. pumilus II was cultured at $28^{circ}C$ for 60 hours. The production of enzyme by B. subtilis IT was maximal as grown on the medium, containing of xylan $2.0\%,;yeast; extract;0.6\%,;K_2HPO_4;0.1\%;and;ZnSO_4;0.04\%$ at pH 8.0 and $34^{circ}C$ for 36 hours. The activities of FPase and xylanase in tested 4 strains were not much different with Thermomonospora fusca.