Enzymatic pulping properties of mixed office waste paper in standard disintegrator were investigated for successful enzymatic deinking of mixed office waste paper. Enzymatic pulping need more revolution in standard disintegrator than alkaline pulping and Cellusoft need more revolution than Denimax. The freeness of disintegrated pulp with enzyme was higher than those of disintegrated pulps with alkaline and heat killed enzyme. The freeness of disintegrated pulp with Denimax was higher than that of disintegrated pulp with Cellusoft. The freeness of disintegrated pulps were increased with a dosage of enzymes. The mechanical properties of disintegrated pulp were improved with enzyme addition comparing with heat killed enzyme. The tensile and burst index of hand sheet of disintegrated pulps with acidic Cellusoft were higher than that of others.