Purpose: This study was done to compare the effectiveness of web learning versus traditional education for learning Asepsis technique in Basic Nursing Practice. Method: This research was a quasi-experimental study. The participants were 60 students who were taking Basic Nursing at a nursing college in G city. Thirty students each were assigned to the experimental and the control group. Data were collected between September 26 and 30, 2005, The data were analyzed using descriptive statistics, Independent t-test and ANCOVA Results: The mean score for competence in Asepsis technique practice in the experimental group was 24.73 and in the control group 22.80. This result was statistically significant(t = 3.723, P = .001). The mean score for knowledge in the experimental group was 42.93 and in the control group 38.93. This result was statistically significant(t = 2.119, P = .038). The mean score for satisfaction with degree of study in the experimental group was 55.50 for the pre-test and 56.17 for the post-test, and in the control group 60.23 and 58.00 respectively. This result was statistically significant(F = 10.537, P = .002). Conclusion: The Web based learning was found to be effective in nursing practice, student satisfaction and knowledge, but web-based programs have to be continuously managed and various demands from learners have to be accepted. Eventually we have to use web-based program as educational medium to promote effective learning nursing education.