This study was conducted to examine the growth and physiological characteristics of Polystichum lepidocaulon native fern as affected by soil mixture as an environment modeled on habitate where was sunken-condition. 1. Polystichum lepidocaulon grew well sunken more than non-sunken condition. Under soil mixture of field soil : sand : leaf mold, Plant height, frond width, frond length, stipe length and ornamental value were increased compared with the other soil mixture. 2. Fresh and dry weight of fronds were higher with non-sunken than sunken condition. In sunken condition, fresh and dry weight were better with field soil : sand : leaf mold than the other soil mixture. 3. Number of spore fronds were increased with sunken condition. As sunken condition, sand : leaf mold was better than field soil : sand : leaf mold or leaf mold. 4. Photosynthetic rate, $CO_2$ absorption rate and water efficiency were higher with field soil : sand : leaf mold than that of sand : leaf mold or leaf mold. expect of stomatal conduction and $CO_2$ use efficiency.