Objectives : This study was performed to observe effect of acupuncture stimulation on heart rate variability in stroke patients. Methods : Data were obtained from heart rate variability of 29 stroke patients. Heart rate variability was measured total five times(before and after needle insertion, before and after needle removal, and 10 minutes after needle removal) for five minutes each time. Results : Heart rates were continuously decreased until 10 minutes after needle removal. SDNN, Total power, LF, and HF were increased until 5 minutes after needle removal, but deceased between 5 and 10 minutes after needle removal. There was no significant changes between male and female and among age groups. Conclusion : This study showed that acupuncture stimulation could effect on heart rate variability in stroke patients. Heart rate variability could be a objective tool to verify the mechanism of acupuncture effect in various condition.