Objective : The purpose of this study is to learn the effects of Musimgigong supine position Doyin-therapy (MGSDT) on the autonomous nervous system (ANS) and musculoskeletal system (MSS), and to know how much MGSDT is useful for relaxing the human body. Methods : Eight oriental medical doctors participated in the experiment, who have trained Musimgigong for 6 years or more. Before and after applying MGSDT, we measured BVP/HR, respiration rate, peripheral temperatures, skin conductance, and EMG, using Procomp Infinity and Biograph Infinity of Thought Technology Ltd Results and Conclusions : 1. After the treatment, the participants in MGSDT group reported that they felt refreshed and relaxed. 2 By analyzing the measured data, we observed significant difference in respiration rate(p=.026<.05) and SC(p=.013<.05) before and after applying MGSDT. We also observed relaxing effect in BVP/HR, EMG, but was not significant. On the other hand Temp was stressed but was not significant. Conclusion is that MGSDT can be considered to have an effect on relaxing ANS. Therefore MGSDT could have useful clinical effect to autonomic imbalance.