The present study was designed to investigate the effects of Ligustici Rhizoma on the expression of genes in the pain model induced by acetic acid. cDNA microarray (GenePlorer TwinChipTM Mouse 7.4K) was used to evaluate the gene expressions. The expressions of 32 genes were up-regulated in the Ligustici Rhizoma-treated group: they include the genes coding Casp6, Hrh3, Basp1, Sprr2h, Zfp131, Copz2, LOC432436, Itpr5, etc. The expressions of 16 genes were down-regulated in the Ligustici Rhizoma-treated group: they include the genes coding Il16, Zfpm1, Cacna2d1, Xpo7, Smpdl3b, Dscr1, Harp, etc. The conclusion is that the expressions of 32 genes were up-regulated and the expressions of 16 genes were down-regulated in Ligustici Rhizoma-treated group.