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Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants
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  • Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants
  • Rapid Propagation of Pelagonium Inquinans Via Organogenesis from Mature Leaf Explants
저자명
Hwang. Sung-Jin
간행물명
韓國藥用作物學會誌
권/호정보
2006년|14권 2호|pp.92-95 (4 pages)
발행정보
한국약용작물학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

A method for plant regeneration via organogenesis from Pelagonium inquinans leaf disc has been developed. Mature leaf explants were collected from field-grown plants and used for the induction of adventitious shoot regeneration on Murashige and Skoog (MS) medium supplemented with 3% (w/v) sucrose plus plant growth regulators. Maximum shoot organogenesis, with $11.8{pm}1.5$ shoots (98.6%) per leaf disc, was obtained with $2;mg/l$ $N^6-benzyladenine$ (BA) and $0.5;mg/l$ ${alpha}-naphthyleneacetic$ acid (NAA) in 30 days. For rooting, the in vitro proliferated and elongated shoots were excised into 1.5-2 cm in length microcutting, which were plated individually on an half-strength MS (1/2MS) medium supplemented with 2% (w/v) sucrose plus various concentrations of indole-3-butyric acid (IBA). Shoots rooted with a frequency of 100% following culture on 1/2MS medium containing $0.5;mg/l$ IBA.