In order to evaluate variation of fatigue life of mechanical components including engineering plastics, it is important to estimate probabilistic strain-life curves to accurately define the variation of fatigue characteristics. This paper intends to provide new assessment of P-$varepsilon$-N (probabilistic strain-life curves) for considering the variation of fatigue characteristics in polyacetal. The fatigue strain controlled tests were conducted under constant 50% humidity and room temperature condition by a universal testing machine at strain ratio, R=0. A practical procedure is introduced to evaluate probabilistic strain-life curves. Three probabilistic distributions were used for generating P-$varepsilon$-N curves such as normal, 2-parameter and 3-parameter Weibull. In this study, 3-parameter Weibull distribution was found to be most appropriate among assumed distributions when the probability distributions of the fatigue characteristic were examined using chi-square and Kolmogorov-Smirnov test. The more appropriate P-$varepsilon$-N curves for these materials are generated by the proposed method considering 3-parameter Weibull distribution.