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Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
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  • Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
  • Determination of Abamectin Residue in Paprika by High-Performance Liquid Chromatography
저자명
Xie. Wen-Ming,Ko. Kwang-Yong,Kim. Sung-Hun,Chang. Hee-Ra,Lee. Kyu-Seung
간행물명
한국환경농학회지
권/호정보
2006년|25권 4호|pp.359-364 (6 pages)
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한국환경농학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Reversed-phase high-performance liquid chromatography (HPLC) techniques were developed to quantify abamectin (ABM) in paprika (Capsicum annum). Separation was achieved on a $C_{18}$ ODS column with a mobile phase of acetonitrile/water (96/4, v/v) mixture in an isocratic elution at the flow tate of 1.2 mL/min for avermectins (AVMs). The retention times were 8.0 and 9.7mins for AVM $B_{lb}$ and AVM $B_{1a}$, respectively. Residual AVMs (sum of AVM $B_{1a}$, AVM $B_{1b}$ and 8,9-Z-AVM $B_{1a}$) in the vegetable were extracted with acetonitrile, and the silica solid-phase extraction cartridges were used to purify the extract. AVMs were derivatized using trifluoroacetic acid and 1-methylimidazole, and the derivatives were determined with a fluorescence detector (excitation at 365 nm and emission at 470 nm). High and consistent recoveries, ranging from 93% to 115%, were obtained for AVM $B_{1a}$ and 8, 9-Z-AVM $B_{1a}$ at fortified levels of $20{mu}g/kg;and;200{mu}g/kg$ for paprika. The limit of quantitation (LOQ) was $2{mu}g/kg$. The residual levels of AVMs in paprika in a field experiment from one day to seven days after the last application decreased from 18.40 to $7.59{mu}g/kg$. The half-life $(T_{1/2})$ of AVMs in paprika was 1.47 days.