Mixing vanes have been installed in the space grid of nuclear fuel rod bundle to improve turbulent heat transfer. Split mixing vanes induce the vortex flow in the cooling water to swirl in sub-channel of fuel assembly. But, The swirling flow decays rapidly so that the heat transfer enhancing effect limited to short length after the mixing vane. In thi present study, the large scale vortex flow(LSVF) is generated by rearranging the mixing vanes to the coordinated directions. This LSVF mixing vanes generate the most strong secondary flow vortices which maintain about 35 $D_H$ after the spacer grid. The streamwise vorticity generated by LSVF sustain two times more than that split mixing vane. Heat transfer in the rod bundle occurs greatly at the same direction to cross flow, and maximum temperature at the surface of bundle drops about 1.5K