In order to develop tools for an early serodiagnosis of Plasmodium falciparum infection, we evaluated the usefulness of P. falciparum liver stage antigen-3 (LSA-3) as a serodiagnostic antigen. A portion of LSA-3 gene was cloned, and its recombinant protein (rLSA-3) was expressed in Escherichia coli and purified by column chromatography. The purified rLSA-3 and 120 test blood/serum samples collected from inhabitants in malaria-endemic areas of Mandalay, Myanmar were used for this study. In microscopic examinations of blood samples, P. falciparum positive rate was $39.1\%$ (47/120) in thin smear trials, and $33.3\%$ (40/120) in thick smear trials. Although the positive rate associated with the rLSA-3 $(30.8\%)$ was lower than that of the blood stage antigens $(70.8\%)$, rLSA-3 based enzyme-linked immunosorbent assay could detect 12 seropositive cases $(10.0\%),$ in which blood stage antigens were not detected. These results indicate that the LSA-3 is a useful antigen for an early serodiagnosis of P. falciparum infection.