A new calibration method for five-hole pressure probe is presented. This method provides accuracies better than those based on the traditional regression method. The calibration algorithm uses a single sector interpolation response surface calculated by comparing the regression curve fits with the actual calibration data. A five-hole pressure probe with hemispherical tip was fabricated and calibrated at Reynolds number of 4.11×106/m and flow angle of ±48 degrees. Two data prediction models, the least-square regression and a single sector error interpolation, were evaluated. The comparison of these two calibration methods to a five-hole probe is described and discussed. An evaluation of the calibration accuracy is also given.