The micromechanical approach was used to investigate the interfacial strain distributions of a unidirectional composite under transverse loading in which fibers were usually found to be randomly packed. Representative volume elements (RVE) for the analysis were composed of both regular fiber arrays such as a square array and a hexagonal array, and a random fiber array. The finite element analysis was performed to analyze the normal, tangential and shear strains at the interface. Due to the periodic characteristics of the strain distributions at the interface, the Fourier series approximation with proper coefficients was utilized to evaluate the strain distributions at the interface for the regular and random fiber arrays with respect to fiber volume fractions. From the analysis, it was found that the random arrangement of fibers had a significant influence on the strain distribution at the interface, and the strain distribution in the regular fiber arrays was one of special cases of that in the random fiber array.