In this paper, we proposed a location estimation algorithm considering an extension of searching area in 2.45GHz band RTLS and analyzed its performance in terms of an average estimation error distance. The extendable searching area was assumed to be square of $300m{ imes}300m$ and 2 dimensions. The arrangement shape of available readers was considered circle, rectangle, and shrinkage rectangle for extendable searching area. Also, we assumed that propagation path was LOS (Line-Of-Sight) environment, and analyzed the estimation error performance as a function of the number of received sub-blink considering an arrangement shape of available readers in searching area. From the results, compared with rectangle shape, circle shape showed the higher estimation accuracy. Also, we confirmed that the proposed location estimation algorithm provided high estimation accuracy in the shrinkage rectangle shape that was suitable for extension of searching area.