DFDBA(Decalcified freeze-dried bone allograft) is one of the allograft materials for periodontal bone regeneration. DFDBA provides an osteoconductive surface and osteoinductive factors. Therefore, DFDBA have been used successfully to regenerate the attachment apparatus during periodontal treatment. But recent studies was reported that wide variations in commercial bone bank preparations of DFDBA do exist, including the ability to induce new bone formation. DFDBA was experimental materials that was recovered, processed, tested, shipped and invoiced through Musculoskeletal Transplant Foundation. MTF(Musculoskeletal Transplant Foundation) is the world largest, non-profit, AATB(American Association of Tissue Banks) accredited tissue bank. The objective of this study was to determine the effects of serial dilutions of a DFDBA on human fetal osteoblastic cell proliferation and their potential to form and mineralize bone nodules. Human fetal osteoblastic cell line(hFOB 1.19) was cultured with DMEM and SSE($1{mu}g/m{ell}$,$10{mu}g/m{ell}$, $100{mu}g/m{ell}$, $1mg/m{ell}$) at $34^{circ}C$ with 5% CO2 in 100% humidity. Cell proliferation was significantly increased at $1mg/m{ell}$, $100{mu}g$, $10{mu}g/m{ell}$, $1{mu}g/m{ell}$, $100ng/m{ell}$, $10ng/m{ell}$, $1ng/m{ell}$ of DFDBA after 5 days incubation (p<0.05). Alkaline Phosphatase(ALP) level was significantly increased in $100ng/m{ell}$, $10ng/m{ell}$, $1ng/m{ell}$ of DFDABA(p<0.05). A quantified calcium accumulation was significantly increased at $1ng/m{ell}$, $10ng/m{ell}$ of MTF(p<0.05). These results indicated that DFDBA has an inductive effect on bone formation in vitro.