The major objective of this study is to investigate the effects and application of external post-tensioning method far steel plate girder bridge. It analyzed the mechanical behaviors of steel plate girder bridge with applying external post-tensioning on the finite element analysis, field test and laboratory test fur the lateral dynamic characteristics. As a result, the reinforcement of steel plate girder bridge the external post-tensioning method are obviously effective for the lateral dynamic response which is non-reinforced. The analytical and experimental study are carried out to investigate the post-tension force decrease lateral acceleration and deflection on steel plate girder bridge for serviceability. And the external post-tensioning method reduce dynamic maximum displacement(about $10{sim}24%$), the increase of dynamic safety is predicted by adopting external post-tensioning method. From the dynamic test results of the servicing steel plate girder bridge, it is investigated that the change degree of natural frequency is very low with applying the external post-tensioning method The servicing steel plate girder bridge with external post-tensioning has need of the reasonable reinforcement measures which could be reducing the effect of lateral dynamic behavior that degradation phenomenon of structure by an unusual response characteristic and a drop durability.