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Characteristics of Transformed Panax ginseng C.A. Meyer Hairy Roots: Growth and Nutrient Profile
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  • Characteristics of Transformed Panax ginseng C.A. Meyer Hairy Roots: Growth and Nutrient Profile
  • Characteristics of Transformed Panax ginseng C.A. Meyer Hairy Roots: Growth and Nutrient Profile
저자명
Jeong. Gwi-Taek,Park. Don-Hee
간행물명
Biotechnology and bioprocess engineering
권/호정보
2006년|11권 1호|pp.43-47 (5 pages)
발행정보
한국생물공학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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기타언어초록

Ginseng (Panax ginseng CA. Meyer) hairy root cultures, which are established via the infection of ginseng root discs with Rhizobium rhizogenes, have been used to construct profiles of both biomass growth and nutrient consumption in flask cultures. In a 250 mL shake flask culture, the maximum biomass was observed on the 59th day of the culture period, at 216.8 g (fresh wt) per liter or 11.4 g (dry wt) per liter. The hairy roots were determined to have a growth rate of 0.355 g-DW/g cells/day during the exponential growth phase and a maximum specific growth rate on day 7. Total ginseng saponin and phenolic compound contents were noted to have increased within the latter portion of the culture period. Linear correlations between increases in biomass weight and nutrient uptake were used to imply the conductivity yield $2.60g-DW/(L{cdot}mS)$ and carbon yield 0.45 g-DW/(g sugar) in the 250 mL flask cultures. The biomass yield when two different nitrogen sources were used (ammonia and nitrate) was shown to remain approximately constant. at $0.47g-DW/(L{cdot}mM;NH_4$) and $0.33g-DW/(L{cdot}mM;NO_3$); it remained at these levels for 16 days with the ammonia. and for 24 days with the nitrate. The biomass yield when a phosphate source was used was also shown to remain approximately constant for 9 days, at $3.17g-DW/(L{cdot}mM;PO_4$), with an $R^2$ of 0.99.