High speed PC-based DIO(Digital I/O) system that consists of a master device and slave I/O devices is developed. The PCI interfaced master device controls all of serial communications, reducing the load on the CPU to a minimum. The slave device is connected from the master device and another slave device is connected to the slave device, it can repeated to maximum 64 slave devices. The slave device has 3 types I/O mode, such as 16 bits input-only, 16 bits output-only, and 8bits input-output. The master device has 2 rings which can take 64 slaves each. Therefore, total I/O points covered by the master is 2048 points. The slave features 3 types of input/output function interchangeability by DIP switch settings. Library, application, and device driver software for the DIO system that have a secure and a convenient functionality are developed.