An accurate knowledge of the AITs(autoignition temperatures) is important in developing appropriate prevention and control measures in industrial fire protection. The measurement of AITs are dependent upon many factors, namely initial temperature, pressure, vessel size, fuel/air stoichiometry, catalyst, concentration of vapor, ignition delay time. The values of the AITs used process safety are normally the lowest reported, to provide the greatest margin of sefety. This study measured the AITs of o-xylene+n-pentanol system from ignition delay time by using ASTM E659-78 apparatus. The experimental AITs of o-xylene and n-pentanol were $480^{circ}C;and;285^{circ}C$, respectively. The experiment AITs of o-xylene+n-pentanol system were a good agreement with the calculated AITs by the proposed equations with a few A.A.D.(average absolute deviation).