In this study, physical/chemical characteristics of the fabrics dyed using Japanese pagoda tree which is a yellowish dyestuff was investigated. We tried to estimate the dyeing mechanism among fiber macromolecules, mordants, chitosan, and dyestuffs by measuring the change of air-permeability according to the dyeing process. Also wash fastness and light fastness were measured in order to estimate the fastness according to the dyeing characteristics. When the chitosan pre-treatment was introduced, the wash fastness increased significantly in the cotton fabrics while the fastness did not change in the nylon fabrics significantly. The light fastness was not improved by the chitosan treatment. when the antibacterial activity was measured for the dyed cotton fabrics, Japanese pagoda tree itself did not exhibit antibacterial activity. However, chitosan treated dyed fabrics exhibited low antimicrobial activity.