The stability and control derivatives of DURUMI-lI UAV using the flight test are obtained. The flight test data is gathered from the normal flight condition (normal mode) and the flight condition assumed as the right elevator fixed (fault mode). Using real-time parameter estimation techniques, applied to Fourier transform regression method, simulates the aircraft motion. From the result, the fault of control surface is to be detected. In this paper, the results of the real- time parameter estimation techniques are compared with the results of the Advanced Aircraft Analysis (AAA). Using the aerodynamic derivatives, it provides the base line of normal/failure for the control surface by using the on-line parameter estimation of Fourier transform regression. In flight, this approach maybe helpful to detect and isolate the fault of primary control surface. It is explained how to perform the flight condition assumed as the right elevator fixed in the flight test. Also, it is mentioned how to switch between the normal flight condition and the assumed fault flight condition.