Many important properties in colloidal systems are usually determined by surface charge $({zeta}-potential)$ of the contacted solid surface. In this study, ${zeta}-potential$ of glass ${mu}-channel$ was evaluated from the electro-osmotic velocity distribution. The electro-osmotic velocity inside a glass f-channel was measured using a micro-PIV velocity field measurement technique. This evaluation method is more simple and easy to approach, compared with the traditional streaming potential technique. The ${zeta}-potential$ in the glass ${mu}-channel$ was measured fur two different mole NaCl solutions. The effect of an anion surfactant, sodium dodecyl sulphate (SDS), on the electro-osmotic velocity and f-potential in the glass surface was also studied. In the range of $0{sim}6mM$, the surfactant SDS was added to NaCl solution in few different mole concentrations. As a result, the addition of SDS increases ${zeta}-potential$ in the surface of the glass ${mu}-channel$. The measured $zeta-potential$ was found to vary from -260 to -70mV. When negatively charged particles were used, the flow direction was opposite compared with that of neutral particles. The ${zeta}-potential$ has a positive sign for the negative particles.