In order to make the way to determine the instream flow more practically, we have investigated many case studies and reviewed reports and papers. To validate instream flow level suggested by the case studies, DAWAST and HEC-RAS model were applied to the Gap-stream watershed in Daejeon city. Flow-duration analysis was performed both with the stream flow data gauged in the Indong, Boksu, and Hoeduck stations, and with the stream flow data estimated by the DAWAST model and the specific discharge method. Instream flow was determined among the flow-duration analysis, DAWAST, HEC-RAS model and mass balance approach. It was satisfied with various factors such as target water quality, water depth for eco-system and resorts, water surface width, flow velocity for landscape in dry season. The study suggested that the mean low flow could be replaced into the instream flow for the preliminary study because the instream flow considering ecosystem, landscape, water-friendly environment and water quality was generally close to the mean low flow.