본 연구에서는 차조기의 30, 50, 70, 95% 에탄올 및 물추출물과 분획물의 전자 공여능, 지질산화 억제능, SOD 유사활성 및 xanthin oxidase 저해 활성을 측정하였다. 추출물의 전자 공여능은 $18{sim}30%$ 정도로 낮았으나, 분획물에서는 95%과 70% 에탄올 추출물의 chloroform 분획이 50% 수준의 활성을 보였고, ethyl acetate 분획에서는 모든 추출용매에서 75% 이상의 활성을 보였다. 특히, 70% 에탄올 추출물의 ethyl acetate 분획물이 93.4%로 가장 높은 활성을 보였으며 $RC_{50}$에 요구되는 농도는 200.5 ppm이었다. 저장 온도 $40^{circ}C$에서 linoleic acid의 자동산화를 가장 효과적으로 억제하는 것으로 나타난 추출물은 70% 에탄올 이었다. 분획물의 경우, 95% 에탄추출물은 hexane과 ethyl acetate 가용성분이 그 외 에탄올 추출과 물추출은 chloroform과 ethyl acetate 가용성분이 가장 높은 억제효과를 나타내었다. SOD 유사활성은 에탄올 추출물이 $28{sim}32%$, 물추출물이 30.3%이었으며, 70% 에탄올 추출물이 32.4%로 가장 높았다. 분획물에서는 70% 에탄올 추출물의 ethyl acetate 가용분이 38.8%로 활성이 가장 높았으며 $IC_{50}$에 요구되는 농도는 1549.0ppm이었다. Xanthine oxidase 저해 활성은 에탄올추출물이 $36{sim}41.2%$이었으며, 물추출물이 46.9%로 추출물중 가장 높았다. 분획물에서는 물추출의 ethyl acetate 가용분이 1,000 ppm 농도에서 52%의 활성을 보여 가장 높았다.
This study was conducted to examine antioxidant activities of Perilla frutescens var. acuta leaf. For the this purpose, DPPH radical scavenging activity, lipid oxidation inhibition, SOD-like activity, and xanthine oxidase inhibitor activity of water extract, ethanol extracts (30, 50, 70, and 95%) and the fractions obtained from these extracts were determined. The electron donating abilities of the chloroform fraction obtained from the 70% and 95% ethanol extracts were 50%, and that of the ethyl acetate fraction for all of the extracts was above 75%. In particular, the electron donating ability of the ethyl acetate fraction of the 70% ethanol extract showed the greatest activity with 200.5 ppm of $RC_{50}$ value. The 70% ethanol extract was most effective to inhibit the automatic oxidation of linoleic acid at $40^{circ}C$ storage. The highest inhibition effects appeared in the chloroform and ethyl acetate fractions of the water extract, and the 30, 50, and 70% ethanol extracts, and the highest lipid oxidation inhibiting effect of the 95% ethanol extract occurred in the hexane and acetate fractions. The SOD-like activity of the water extract was 30.3%, and the activities of the various concentration of ethanol extracts were 28-32% and the activity of the 70% ethanol extract was the highest. The SOD-like activity of the ethyl acetate fraction of the 70% ethanol extract was highest with 1,549.0 ppm of $RC_{50}$ value. Xanthine oxidase inhibition activity was greatest in the water extract and the activities of the ethanol extracts were 36-41.2%. The xanthine oxidase inhibition activity of the ethyl acetate fraction of the water extract was highest. In summary, we found that electron donating ability, lipid oxidation inhibition, and SOD-like activity of Perilla frutescens var. acuta leaf were greatest in the ethyl acetate fraction of the 70% ethanol extract, and xanthine oxidase inhibition activity was highest in the ethyl acetate fraction of the water extract.
