Injection molding process factors such as molding temperature, injection pressure, flow rate and flow velocity, must be controlled properly in filling and packing phases in the injection molding process. In this study, effects of these factors on the injection molding were investigated through the flow analysis for birefringence and refractive index for pickup lens. This paper presents the birefringence and refractive index reduced with increasing the holding pressure and also the holding pressure time. And there are interaction with birefringence and fill time in the injection process. The optimal conditions through DOE are validated by using injection molding analysis.