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Protection of Polaromonas naphthalenivorans CJ2 from Naphthalene Toxicity by Extracellular Polysaccharide Capsules
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  • Protection of Polaromonas naphthalenivorans CJ2 from Naphthalene Toxicity by Extracellular Polysaccharide Capsules
  • Protection of Polaromonas naphthalenivorans CJ2 from Naphthalene Toxicity by Extracellular Polysaccharide Capsules
저자명
Park. Min-Jeong,Jeon. Ye-Ji,Madsen. Eugene L.,Jeon. Che-Ok
간행물명
Journal of applied biological chemistry
권/호정보
2007년|50권 2호|pp.41-45 (5 pages)
발행정보
한국응용생명화학회
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정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
서지반출

기타언어초록

Polaromonas naphthalenivorans CJ2, responsible for naphthalene degradation at a coal tar contaminated site, was isolated on MSB agar media supplied with naphthalene vapor as the sole carbon source at $10^{circ}C$. The strain is not isolated under the same isolation condition using the same soil sediment at $20^{circ}C$ although its optimum temperature is about $20^{circ}C$. In this work we explored the reason why strain CJ2 could not have been isolated on MSB agar with naphthalene vapor at $20^{circ}C$. Dispersed CJ2 cells in PBS buffer formed colonies on MSB agar with naphthalene vapor at $10^{circ}C$ with low naphthalene vapor pressure, but not at $20^{circ}C$ with high naphthalene vapor pressure. However, streaked cells without resuspension grew on MSB agar with naphthalene vapor at $10^{circ}C,;20^{circ}C$, and even $25^{circ}C$. Investigation of scanning electron microscopy showed that CJ2 cells formed extracellular polysaccharide (EPS) capsules, which were released easily from CJ2 cells by just dispersion. Therefore, it is concluded that strain CJ2 is able to overcome the naphthalene toxicity by forming a capsule-type barrier around the cells although it is susceptible to naphthalene toxicity at high temperature.