8-Hydroxydeoxyguanosine $(oh^8dG)$ treatment induced senescence-like changes in KG-1 cells, a human acute myelocytic leukemia cell line. The $oh^8dG-treated$ cells stained positive for senescence associated ${eta}-galactosidase$ $(SA-{eta}-galactosidase)$ and had enlarged cell shape, both of which are senescence indexes. The $oh^8dG-treated$ cells were also cell growth inhibited and arrested at $G_1$ in the cell cycle. The accumulation of cdk (cyclin dependent kinase) inhibitors, such as p16, p21, and p27, also implies that cellular senescence was induced in $oh^8dG-treated$ cells. However, these changes were not accompanied by cell differentiation or telomerase activity. Taken together, we conclude that $oh^8dG$ treatment of KG-1 cells induces cellular senescence.