Objectives : The present study purposed to compare the anti-oxidative effect of Fructificatio Inonoti Obliqui aqueous extract according to extraction temperature. Methods : We measured the total phenol content and anti-oxidative activity of Fructificatio Inonoti Obliqui total extract and $50^{circ}C$ low-temperature leachate. Results : Anti-oxidative activity of Fructificatio Inonoti Obliqui total extract and $50^{circ}C$ low-temperature leachate was different according to extraction temperature, concentration, and antioxidant effect measuring method. In all experiments, the total extract showed higher antioxidant than n-BuOH fraction. The total phenol content was higher in Fructificatio Inonoti Obliqui total extract than $50^{circ}C$ low-temperature leachate. Conclusions : Both Fructificatio Inonoti Obliqui total extract and Fructificatio Inonoti Obliqui $50^{circ}C$ low-temperature leachate have significant anti-oxidative effect.