Purpose: This study was to examine the effects of the Danjeon Breathing Exercise Program on physical fitness, levels of serum lipid and physical symptom of people using public health center at Yangju city. Method: One group pretest-posttest design was utilized for this study. This study has been done from July 6 to September 28, 2005 and the subjects of the study were 28 persons. Danjeon breathing exercise was carried out for 60 minutes a day, 3 times a week for 12 weeks. Physical fitness was measured using the Health Management System developed by the Korea Physical Science Institution. Serum lipid level was measured in public health center at Yangju city. Questions of physical symptom (35 items) were chosen from Cornell Medical Index. The collected data were processed by using the SPSS PC program and analyzed by using Wilcoxon Signed Rank Test. Result: 1. The scores of back strength, flexibility and balance of post-test were more increased after Danjeon breathing exercise than those of pre-test. But the score of muscular endurance of post-test was not significantly increased. 2. The degrees of total cholesterol, LDL-cholesterol and triglyceride of post-test were more decreased after Danjeon breathing exercise than those of pre-test. But the degree of HDL-cholesterol of post-test was not significantly increased in statistics. 3. The score of physical symptoms of post-test was not significantly decreased in statistics after Danjeon breathing exercise than that of pre-test. Conclusion: It is concluded that the Danjeon Breathing Exercise Program could increase the physical fitness and decrease levels of serum lipid. So Danjeon breathing exercise could be utilized to promote physical fittness and to decrease level of serun lipids.