반응표면분석법을 이용하여 생강의 추출조건에 따른 기능성을 모니터링하였다. 중심합성계획법에 따라 마이크로웨이브 에너지($30{sim}150;watt$), 에탄올 농도($0{sim}100%$), 추출시간($1{sim}9$분)을 달리하였을 때 회귀식 $R^{2}$는 추출수율, 전자공여작용, tyrosinase 저해효과 및 polyphenol 함량이 각각 0.9152, 0.9239, 0.9696과 0.9057로 분석되었다. 조건별 추출물의 수율, 전자공여능, tyrosinase 저해효과, polyphenol에 대한 4차원 반응표면을 superimposing하여 얻은 최적 추출조건 범위는 마이크로웨이브 에너지 $0{sim}80;watt$, 에탄올 농도 $0{sim}40%$, 추출시간 $3{sim}7$분으로 나타났다. 최적 추출조건 범위 내의 임의의 조건인 마이크로웨이브 에너지 50 watt, 에탄올 농도 20%, 추출시간 5분을 각각의 회귀식에 대입하여 얻은 예측값은 추출물의 수율 22.92%, 전자공여능 35.76%, tyrosinase 저해효과 56.52%, polyphenol 함량은 37.68%로 나타났다.
Extraction characteristics of ginger (Zingiber officinale) and the functional properties of the corresponding extracts were monitored by the response surface methodology (RSM). The maximum extraction yield of 38.83% was obtained under the specific extraction conditions, such as microwave power of 126.68 W, ethanol concentration of 53.39% and extraction time of 8.15 min. The maximum electron donating ability (EDA) was found as 77.92% at the conditions of 58.25 W microwave power, 91.87% ethanol concentration and 5.54 min extraction time. The maximum inhibitory effect on tyrosinase was 79.39% at 106.08 W, 2.06% and 5.61 min. The maximum polyphenol content was 105.59% under the extraction conditions of 81.82 W, 99.52% and 4.06 min. Based on superimposition of four dimensional RSM with respect to extraction yield and electron donating ability and polyphenol content obtained under the various extraction conditions, the optimum ranges of extraction conditions were found to be microwave power of $0{sim}80;W$, ethanol concentration of $0{sim}40%$ and extraction time of $3{sim}7$ min.
