The seed paper was used in farm field recently for a sound young plant. The most of seed paper are made of synthetic non-woven sheet. Therefore, it is very difficult to bio-degrade in soil and is very hard to have some special function, for example keeping herbicide and/or insecticide activity because of its lack of chemical acceptability. The purpose of this research is manufacture of seedling paper which have a function of herbicide activity from waste paper. The fiber properties from waste paper were remarkably improved by fine removal with washing and/or flotation process. The paper-making ability for seed paper was enhanced with enzyme treatment of secondary fibers. The paper for seedling must have a good bio-degradation ability in soils. The absorption amount of chemical like as dithiopyr was increased remarkably in enzyme treated base paper. The embossing treatment of base paper was very effective for seed attachment and chemicals retention. And also, the developed seed paper showed a good penetration property of young root through embossed paper.