The Work-Related Musculoskeletal Disorders (WMSDs) can be occurred by various factors such as repetition, forceful exertions and awkward postures. Especially, occurrences of the WMSDs on the waist and lower limb are reported in workplaces, demanded standing postures for a long time, in service and manufacturing industry. The static and standing postures without movement for a long time increase work loads to the lower limb and the waist. Accordingly, anti-fatigue mat or anti-fatigue insole is used as a preventing device of the WMSDs. However anti-fatigue mats are limited in space and movement. In this study, multi-elastic insoles are designed and shown the effects of the workload reduction for a long time under the standing work. The foot pressures and EMG (Electromyography) are measured at 0 hour and after 2 hours by 6 health students in their twenties. The 6 prototype insoles are designed with three elastic (Low, Medium and High). These insoles are compared with no insole (insole type 7) as control group. The EMG measurement was conducted to waist (erector spinae muscle), thigh (vastus lateralis muscle) and calf (gastrocnemius muscle). The foot pressure is analyzed by mean pressure value and the EMG analysis is investigated through MF (Median Frequency), MPF (Mean Power Frequency) and ZCR (Zero Crossing Rate). The results of the foot pressure show that the multi-elastic insoles had smaller foot pressure value than that of no-insole. Moreover, Insole 2 and Insole 3 have the smallest increasing rate in foot pressure. The EMG results show that the multi-elastic insoles had smaller EMG shift value than that of no-insole in 2 hour, and then shift value shows the smallest value in Insole 2. Therefore, this study presents that the multi-elastic insoles have reducing effects of the work load for a long time standing work in both side of foot pressure and EMG.