The physiological and biochemical responses of healthy men and women to life stress were measured in order to temperature, blood pressure (BP), heart rate (HR) and galvanic skin response (GSR) were selected as physiological stress indices and salivary cortisol level was used as a biochemical stres biomarker. Twenty six (male 14 and female 13) colege students were participated in the experiment. Female showed the significant higher value of salivary cortisol level (p<0.01), diastolic BP (p<0.01), and HR (p<0.01) than male. The difference of skin temperature between forehead and fingertip correlated significantly with salivary cortisol level (p<0.01). The LF(low frequency)/HF(high frequency) ratio of HRV also correlated significantly with salivary cortisol level (p<0.01). However, BP, HR and GSR corelated insignificantly with salivary cortisol level. We suggest that LF/HF ratio of HRV and skin temperature may be good indices for the assessment of life stress, and may apply to measure the stress level of individual in real time.