This study was investigated to know the effect of subzero treatment in austempered ductile cast iron. Retained austenite transformed to martensite by subzero treatment. With decreasing subzero treatment temperature, more volume fraction of retained austenite transformed to martensite and transformed to martensite above 30% by subzero treatment temperature at $-196^{circ}C$. With decreasing subzero treatment temperature, the value of strength and hardness increased but the value of elongation and impact value decreased. In case of subzero treatment at $-196^{circ}C$, hardness value increased about 18% and impact value decreased by above 20%. We could find that subzero treated specimens had a little of effect on the tensile properties but had very much effect on the hardness and value of the impact.