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Antioxidative Effect of Astaxanthin on In Vitro Development of Porcine IVF Embryos
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  • Antioxidative Effect of Astaxanthin on In Vitro Development of Porcine IVF Embryos
  • Antioxidative Effect of Astaxanthin on In Vitro Development of Porcine IVF Embryos
저자명
Jeon. Gi-Jun,Jang. Hyun-Yong,Cheong. Hee-Tae,Kim. Jong-Taek,Park. Choon-Keun,Chu. Duong-Xuan,Lee. Hak-Kyu,Yang. Boo-Keun
간행물명
Reproductive & developmental biology
권/호정보
2007년|31권 1호|pp.49-53 (5 pages)
발행정보
한국동물번식학회
파일정보
정기간행물|ENG|
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이 논문은 한국과학기술정보연구원과 논문 연계를 통해 무료로 제공되는 원문입니다.
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Astaxanthin is a kind of carotenoid compounds, having a antioxidant and anti-inflammatory activities. The antioxidative mechanism by which carotenoid scavenge free radicals has been clearly elucidated, but has not tried for the development of mammalian preimplantation embryo. This study was conducted to investigate the antioxidative effect of astaxanthin on in vitro development of porcine in vitro fertilized embryos. Porcine embryos derrived from in vitro fertilization (IVF) were cultured in 5% $CO_{2} in air at $38.5^{circ}C$ in PZM-3 medium supplemented with different dosages of astaxanthin ($0,;1,;5;and;10{mu}M$) and taurine (0, 1, 2.5 and 5 mM) as a positive control, and execute to compare the effects of various antioxidants such as taurine, melatonin and asculatin on in vitro development. The proportions of embryos developed to the blastocyst stage were increased when $1;and;5;{mu}M$ of astaxanthin (26.6 and 23.4%, respectively) and 1 and 2.5 mM taurine (25.8 and 26.4%, Respectively) were supplemented, compared to controls (p<0.05). Also, various antioxidant-treated groups were significantly higher rates of blastocysts (astaxanthin, 27.4%; taurine, 29.1%; melatonin, 26.8%; aesculetin, 27.9%, respectively) than control (18.8%). There was no difference in mean cell number of blastocysts between antioxidants and control. This result indicates that astaxanthin has an antioxidant feature when porcine IVF embryos were cultured in vitro.