본 연구는 효율적인 돼지 난자의 활성화를 통한 수핵란의 대량 확보를 위하여 ethanol, Ca-ionophore 및 strontium의 최적 농도 및 노출 시간을 규명하기 위하여 실시되었다. Ethanol은 10%에서 10분간 노출시켰을 때 난할율과 배발달 성적이 각각 51.4%와 45%로 다른 처리구에 비하여 유의적으로 높았으며 (P<0.05), Ca-ionophore는 $25{mu}M$에서 2분간 노출시켰을 때 난할율과 배발달 성적이 유의적으로 높았다. 또한 strontium은 20mM에서 6시간 노출시켰을 때 난할율과 배발달 성적 모두 유의적으로 높았다(P<0.05). Strontium중복 처리는 단독 및 병용처리 할 때보다 난할율과 배발달율 모두 유의 적으로 낮았다(P<0.05). 이러한 결과는 돼지 난자의 활성화 조건을 확립하기 위한 활성화제의 최적 농도와 노출 시간 확립에 기여할 수 있으리라 생각된다.
The objective of this study was to examine the optimal concentration and the exposure time of ethanol, Ca-ionophore, and strontium to achieve massive recipient oocytes in porcine. The cleavage (51.4% vs. $21.3{sim}44.3%$) and embryo development rates (45% vs. $13.3{sim}29.9%$) were significantly higher (p.0.05) in oocytes treated with 10% ethanol for 10 min than other treatments. The oocytes treated with 25mM Ca-ionophore for a minimum of 2min and 20mM strontium for a minimum of 6h showed significantly higher cleavage and embryo development rates than those of other treatments (P<0.05). Cleavage rate with duplicated ethanol treatment was significantly lower than those with ethanol alone (P<0.05). The cleavage rate and embryo development rates were significantly lower in duplicated strontium treatment than those in both alone and combination (P<0.05). But the cleavage and embryo development rates in treatment with Ca-ionophore were significantly higher in combined treatment (Ca-ionophore and cycloheximide) than those in single or duplicated treatment (P<0.05). These results might induce establishment of the optimal concentration and the exposure time on activation media to build up activation condition of porcine oocytes.
