A new method for competent extraction of safflower (Carthamus tinctorius Linnaeus) colorants by using ultrasonic device was developed. When comparing to general extraction method, the ultrasonic method showed high extraction efficiency of safflower pigments. Ultrasonic method gave a higher extraction yield of red and yellow safflower pigments than using general method. It is supposed that the extraction efficiency is to be attributed to high vibration energies from ultrasound and finally induced physical changes of the pigments. Furthermore, this study explored the effects of ultrasonic treatment into the extracted safflower pigments on dyeing of cotton fabrics. Ultrasonic treatment into the extracted pigments exhibited significantly improved dyeing properties for the cotton fabrics.