진세노사이드 Rd가 지방세포분화에 미치는 영향을 관찰한 결과 다음과 같은 결론을 얻었다. 1. 진세노사이드 Rd는 3T-L1지방세포모델에 있어 효과적으로 지방분화를 억제한다. 2. 진세노사이드 Rd는 세포내 에너지대사의 필수 단백질인 AMPK를 활성화시키고 또한 지방분화과정에 발현 및 활성이 증가하는 PPAR 감마의 활성을 효과적으로 억제한다. 이상의 결과로 진세노사이드 Rd는 세포내 에너지대사를 촉진하여 지방축적 억제에 탁월한 효과를 보일 것으로 사료된다.
Obesity is a major obstacle for human health, which induces various diseases such as cardiac injury and type 2 diabetes. Ginsenosides, active components of ginseng extract, exert various physiological effects. However, There are still no evidence for their anti obesity effects. In this study, we investigated the effects of ginsenoside Rd on adipocyte differentiation in 3T3-L1 cells. Our data show that ginsenoside Rd (80 uM) was effective in adipocyte differentiation inhibition. These inhibitory effects of ginsenosides on adipocyte differentiation were accompanied by PPAR gamma inhibition in rosiglitazone-treated cells. We also tested whether AMP-activated protein kinase (AMPK) activation was involved in the effects of these ginsenosides. AMPK is a master target for obesity, ginsenoside Rd significantly activated AMPK. Taken together, these results suggest that the anti obesity effects of ginsenoside Rd involve the AMPK signaling pathway and PPAR-gamma inhibition.
