본 연구에서는 수성이상계에서 Pluronic F-68과 산소전달물질을 적용하여, 물질전달과 산소전달을 증가시켜 당귀세포의 증식을 향상시켰다. 특히 산소전달물질인 n-hexadecane이 Pluronic F-68보다 수성이상계에서의 당귀 세포증식에 더 긍정적임을 확인하였다. 따라서 Pluronic F-68과 적절한 산소 전달물질의 첨가는 수성이상계 뿐만 아니라 대량배양을 위한 고농도배양 등에 효과적으로 적용가능하리라 사료된다.
Pluronic F-68 and oxygen vectors were applied to increase the cell growth of Angelica gigas Nakai in aqueous two-phase system (ATPS). ATPS was composed of 3.6% (w/v) polyethylene glycol (PEG) 20,000 and 2.8% (w/v) crude dextran. n-Hexadecane, n-dodecane and FC-40 were used as oxygen vectors to enhance the oxygen transfer in ATPS. With 2$sim$10 g/L of Pluronic F-68, addition of of n-hexadecane and FC-40 significantly enhanced the oxygen transfer rate as well as the maximum cell mass in a medium with ATPS. However, n-dodecane reduced the cell growth in all treatments. Maximum cell densities were increased up to 27.5% with 10 g/L of Pluronic F-68 and up to 40.2% with 8% (v/v) n-hexadecane compared to those of the controls without Pluronic F-68 and oxygen vectors. It was confirmed that the cell growth could be increased in ATPS using n-hexadecane.
