The importance of information security is increasing by the rapid development of the communication network. So, cryptosystems are used to solve these problems and securities of cryptosystems are dependent on keys. In this paper, we propose a key generation method which is based on cryptographically secure MD5 hash function. The basic structure of the MD5 hash function features is a repetitive structure which is processed in a block unit of 512 bits from inputs of limited length and generates a fixed output of 128 bits. The security of proposed method is based on the hash function and the proposed method can be also utilized for authentication algorithm or data encryption algorithm.