In recent years, the particle number emissions rather than particulate mass emissions in automotive engine have become the subject of controversial discussions. Recent results from the health effects studies imply that it is possible that particulate mass does not properly correlated with the variety of health effects attributed to diesel exhaust. So, the concern is instead now focusing on nano-sized particles emitted from I. C. engine. This study has been performed for the better understanding about the engine nano-particle for 3-measurement systems with different measuring principle. Firstly, EEPS is a newly introduced instrument for size distribution measurement of engine exhaust particles. It can measure nano-particles with an adequate resolution and in real time. In this study, the characteristics of EEPS were compared with ELPI and SMPS. As a research results, EEPS showed a same effect of engine load on the size distribution with ELPI and SMPS. But the quantitative results of EEPS were more similar to SMPS than ELPI, because the EEPS and SMPS use a same principle for classifying particles by size. The capability for transient measurement of EEPS was equivalent to that of ELPI.