The design and synthesis of a state feedback controller assumes the feedback of all state variables of the system. However, some state variables are not physical quantifies so that sensors may not be available, or may be too expensive to measure. Hence, a state observer can be an alternative to estimate unmeasurable state variables. This paper therefore presents a scheme for state observer-based stabilization control of inverted pendulum systems. The feedback gain matrices of both the state feedback controller and the state observer are tuned by real-coded genetic algorithms(RCGAs) such that the given performance indices are minimized. The proposed method is demonstrated through simulations.