The purpose of this study is to verify availability of the acoustic emission in-situ monitoring method to the internal leak and operating conditions of the major valves at nuclear power plants. In this study, acoustic emission tests are performed when the pressurized temperature water and steam flowed through glove valve(main steam dump valve) and check valve(main steam outlet pump check valve) on the normal size of 12 and 18". The valve internal leak monitoring system for practical field was designed. The acoustic emission method was applied to the valves at the site, and the background noise was measured for the abnormal plant condition. To improve the reliability, a judgment of leak on the system was used various factors which are AE parameters, trend analysis, frequency analysis, voltage analysis and amplitude analysis of acoustic signal emitted from the valve operating condition internal leak.