Modern diesel vehicle has to comply with the EURO IV, V regulation with low level of particulate matter and smoke emission Moreover, emission standards of each countries are becoming stringent in advanced countries such as USA and Europe. Because Bio-diesel is similar to diesel fuel, it is essential to judge the environmental and health effects deriving from the use of Bio-diesel in diesel engine. The deterioration characteristics of emission in accordance with aging vehicles must be regulated for Bio-diesel. Therefore, under 1200 driving hours, 220,000km driving distance condition and full load, the deterioration characteristics of emission were estimated. We could reduce sulfur contents of fuel, particulate matter and smoke emission by using Bio-diesel and conform the influence of engine performance, emission, and fuel consumption by Bio-diesel deterioration