This study explored the Eye Movement, the horizontal and vertical flow of vision, and genetic, cultural and empirical schema, and the differentiation of the visual system of the cerebrum through the engineering psychological or the cognitive neuroscientific experiments measuring Eye Movement to maximize the efficiency of the Public Sign System in the urban environment. Accordingly, the study derived an algorithm that can bring out the most efficient visual design measurement by using EMR test and bitmap counting method. The scientific test data in this study will show the existence of the momentum of differentiation and visual sampling by the Schema and Sensory Qualia of the human.