상황버섯 자실체를 80% MeOH 용액으로 추출하고, 추출물을 EtOAc, n-BuOH 및 물로 분배, 추출하였다. 이 중 EtOAc 분획을 silica gel 및 octadecylsilica gel(ODS) column chromatography로 정제하여 4종의 sterol 화합물을 분리하였다. 각 화합물의 화학구조는 NMR, MS 및 IR 등의 스펙트럼 데이터를 해석하여, ergosta-7,24(28)-dien-3${eta}$-ol (episterol, 1), 5${alpha}$,8${alpha}$-epidioxyergosta-6,9(11),22-trien-3${eta}$-ol (dehydroperoxyergosterol, 2), 5${alpha}$,8${alpha}$-epidioxyergosta-6,22-dien-3${eta}$-ol (ergoterol peroxide, 3), 및 $3{eta}$,$5{alpha}$-dihydroxy-6${eta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4)로 동정하였다. 이 화합물들은 이번에 상황에서 처음 분리 보고되었다.
The fruiting body of Phellinus linteus was extracted with 80% aqueous MeOH, and the concentrated extract was partitioned with EtOAc, n-BuOH and $H_2$O. The repeated silica gel and ODS column chromatographies of the EtOAc fraction led to isolation of four sterols. From the result of spectral data including NMR, MS and IR, the chemical structures of the sterols were determined as ergosta-7,24(28)-dien-3${eta}$-ol (episterol, 1), 5${alpha}$,8${alpha}$-epidioxyergosta-6,9(11),22-trien-3${eta}$-ol (dehydrop-eroxyergosterol, 2), 5${alpha}$,8${alpha}$-epidioxyergosta-6,22-dien-3${eta}$-ol (ergoterol peroxide, 3), and $3{eta}$,$5{alpha}$-dihydroxy-6${eta}$-methoxyergosta-7,22-diene (6-O-methylcerevisterol, 4). The ergosterols have been first isolated from this mushroom in this study.
