The liquid chromatographic enantiomer separation of H-fluorenylmethoxycarbonyl (FMOC) protected ${alpha}$-amino acids was performed on nine polysaccharide-derived chiral stationary phases (CSPs). The cellulose derived coated CSPs, Chiralcel OD-H (separation factor = 1.09-2.70) and Chiralcel OD (separation factor = 1.08-2.55), had the best performance of all the CSPs for resolution of H-FMOC ${alpha}$-amino acids and therefore, all analyte enantiomers were base-line separated on Chiralcel OD-H and/or Chiralcel OD. Enantioseparation on cellulosetris(3,5-dimethylpherylcarbamate) derived CSPs (Chiralcel OD-H, Chiralcel OD and Chiralpak IB) is generally greater than that on amylose tris(3,5-dimethylphenylcarbamate) derived CSPs (Chiralpak AD-RH, Chiralpak AD and Chiralpak IA). Additionally, coated type CSPs (Chiralcel OD-H or Chiralcel OD, and Chiralpak AD) generally provided better enantioseparation for these analytes than the covalently bonded type CSPs (Chiralpak IB and Chiralpak IA) with thesame chiral selector of cellulose tris(3,5-dimethylphenylcarbamate) and amylose tris(3,5-dime-thylphenylcarbamate), respectively. However, Chiralpak IB and Chiralpak IA had an advantage over the coated type CSPs in that a broader range of solvents could be used due to itscovalently bonded nature.