This paper is to identify whether the mechanism of the real market as an off-line market developed from the transaction cost theory from previous research is applied to on-line market, such as usiness to Business e-marketplaces. With three factors(parties, product, environments) selected from previous research, four cases picked up from all B2B e-marketplace sites as of April 2007, in the view of Business to Business Matrix which presented by Kaplan & Sawhney(2000), were studied. They were accompanied by interview with CEO and team manager, and they explained their business and revenue model of the company. From the interview, the relation between three factors and usiness to Business e-marketplace was observed by taking sub-factors into consideration; parties -asset specificity, information asymmetry, product factors - standardization, price stability, and environments factors - competition, market uncertainty. The implications of this study are to analyze the relation of the transaction theory in offline and online. Also, this is the first study that analyzed it. In the future, another research based on this research will be studied.