The purpose of this study is to investigate the main factors that affect turnover of Dental Hygienists so that it leads to preventing high turnover and long-term service in their position. Data were obtained from 112 dental hygienists who are currently working for dental clinics from September 17th 2007 to October 16th 2007 via questionnaires. For this study, the demographic factors and unique characteristics of Dental Hygienists are considered as well as all the variables of statistic. The results derived from using SPSS 11.0 are as follows: 1. The average of job related factors is 2.55 point, the average of job satisfaction is 2.59 point, the average of salary related factor is 2.24 point and the average of changing their occupation is 2.85 point. 2. The difference between the factors related to age and changing their occupation shows the highest level on job related factors.(pM0.033) in the age between 26 and 30. 3. Dental Hygienists who are in their occupation for 6years to 9 years answer that salary would be the main reason to change their jobs. 4. For married dental hygienists, salary related factor is the main reason to make them change their occupation.(pM0.002)